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  1. #1
    Join Date
    Mar 2007
    Posts
    1,666

    Best way to light this?

    So havea photo dilemma. I work with a lot of rodent models in my lab and we want to be able to take some pictures of various tissues for a visual comparison in any journal we submit.

    last time i just used my 50mm lens with a close up filter on it to get a pseudo macro shot. worked alright but the lighting for it was really hit or miss. I only have the onboard flash and if i use it, it overexposses everything horribly since the tissues are moist.


    so looking for tips/suggestions on the best way to manage these pictures to get some decent images that we can use.

    what i have

    d70
    20mm
    50mm
    70-210mm
    0, +1, +2 close up filter

    the filter has a 52mm thread which is only on the 50mm lens

    will be needed by next thursday

  2. #2
    Join Date
    Jan 2008
    Location
    Front Range, CO
    Posts
    613
    If you don't want to get an external flash, I would try out a cheap pop-up flash diffuser. I don't have any experience with one, but you could probably build one pretty easily if you don't want to buy it...



    http://www.lumiquest.com/softscreen.htm

    you could also trying bouncing the flash off of a solid white surface.

  3. #3
    Join Date
    Apr 2005
    Location
    Sector 7G
    Posts
    5,693
    This is the worst pain EVER!

  4. #4
    Join Date
    Jul 2006
    Location
    berkeley
    Posts
    1,635
    a white piece of paper over the flash will work fine.

    since you are photographing tissue, white balance is crucial. be sure you are using a gray card every time, otherwise you'll often get an inaccurate representation.

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