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  1. What do I hate most about Corporate America? ...

    What do I hate most about Corporate America?

    The corporations.
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    not seeing a half price option when you need 2...

    not seeing a half price option when you need 2 different liners and 2 different cuffs.
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    ....so 2 different boots then.

    ....so 2 different boots then.
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    Came in off the boat at 2pm and it happened 20min...

    Came in off the boat at 2pm and it happened 20min later one bay over, saw one of the planes in the air but didn't see or hear the collision. Tragic.
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    Coeur d'Alene. 333612333613

    Coeur d'Alene.

    333612333613
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    333600

    333600
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    333399

    333399
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    Which vaccine version did you get Splat?

    Which vaccine version did you get Splat?
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    We are probably set up for outbreaks affecting...

    We are probably set up for outbreaks affecting 5-15% of the population seasonally, but with a portion of those not retaining significant immunity and a higher probability of that occurring with...
  10. Thread: Doggie stoke

    by Mofro261
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    doggie daycare ...

    doggie daycare
    ...
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    My 21yro has been working at Safeway in Bozeman...

    My 21yro has been working at Safeway in Bozeman throughout the lockdown. My 19yro got a job in SLC working at drive up COVID-19 testing locations. At least it's outside. She wants to be a nurse but...
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    No your not getting it. Getting sick or having...

    No your not getting it. Getting sick or having symptoms is actually a result of the body recognizing an infection. In asymptomatics, the body doesn't recognize the virus replicating without causing a...
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    Viral load is generally measurable from a few...

    Viral load is generally measurable from a few days before symptoms appear up to 14 days after. If you dont have symptoms, you dont seek out to get tested. Most asymptomatics are found through...
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    Maybe I didn't understand either after a...

    Maybe I didn't understand either after a undergrad degree in Microbiology. Maybe I didn't understand after getting my PhD in Molecular and Cellular Biology focused on structural biology. Maybe I...
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    its not for CS, it's for those who actually want...

    its not for CS, it's for those who actually want a better understanding.
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    We can measure concentratuon of RNA and not just...

    We can measure concentratuon of RNA and not just its presence, with a lower end detection that reliably falls around 100 virus particles per ml of aquired sample up to 10e8 per ml with one log very...
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    Gee, quick tell me what a CT is and what it means?

    Gee, quick tell me what a CT is and what it means?
  18. Replies
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    I've been focused on diagnostic test development,...

    I've been focused on diagnostic test development, including rtPCR, serology IgM and IgG, as well antigen detection assays. My part is to pick the best targets, define what is the best way to produce...
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    False. RtPCR testing is repeatedly finding just...

    False. RtPCR testing is repeatedly finding just as high a viral load in asymptomatic infection, with prolonged shedding.
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    Point of clarification Lego. Somatic...

    Point of clarification Lego. Somatic hypermutation drives affinity maturation of an antibody leading to an increase in target recognition, not an increase antibody diversity, as the end product of...
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    From epidemiological data, places that have been...

    From epidemiological data, places that have been most effective in reducing the spread of COVID-19 have implemented universal masking, including Taiwan, Japan, Hong Kong, Singapore, and South Korea....
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    Reducing transmission of SARS-CoV-2 Science 26...

    Reducing transmission of SARS-CoV-2
    Science 26 Jun 2020:

    Respiratory infections occur through the transmission of virus-containing droplets (>5 to 10 µm) and aerosols (≤5 µm) exhaled from...
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    let's put this in terms of something you may...

    let's put this in terms of something you may understand, Gasoline. Is gasoline an ingredient in oil? No, it is a product refined from oil. What does refinement do? It removes the impurities....
  24. Watched some crows chasing an eagle carrying a...

    Watched some crows chasing an eagle carrying a baby crow in it's mouth this morning while walking my 9 week old puppy.

    Yes, of course she is the cutest puppy of all time.
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    No, same for those as well. Data from SARS...

    No, same for those as well. Data from SARS neutralizing Ab, again on a small population:

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3298259/bin/04-0906-F3.jpg

    Emerg Infect Dis. 2005...
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    Normally, a neutralization assay involves a...

    Normally, a neutralization assay involves a 2-fold serial dilution of sera mixed with virus (or pseudotyped virus), and several of the other assays I have seen report neutralizing Ab's over several...
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    Couple of issues I had with that study. 1....

    Couple of issues I had with that study.
    1. Serologic markers were a peptide in the C-term of Spike and NP, not the RBD or S1 of Spike, so poor correlates of Ab to protective effect.
    2. Their...
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    The truth has been in front of your nose the...

    The truth has been in front of your nose the entire time, but to quote a famous movie line "you can't handle the truth" because that might involve modifying your own behavior or world view and so...
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    Yes, we call them "pants" ...

    Yes, we call them "pants"

    https://www.forbes.com/sites/brucelee/2020/04/27/can-farts-transmit-covid-19-coronavirus-here-is-what-is-being-said/#33339234310f
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    "It smells like gas when I open the cap, but it...

    "It smells like gas when I open the cap, but it says on the bottle that it's apple juice, so I'll still drink it". And that's being smart?

    Like I said, ignorance will not save you.

    you are...
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    Your own ignorance certainly isn't going to save...

    Your own ignorance certainly isn't going to save you from anything, but maybe it helps you sleep better at night.
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    Since sports and baseball aren't going on, the...

    Since sports and baseball aren't going on, the kids took over the local infield and made bmx jumps. A pack of 10-12yros being kids is all right in my book.
  33. Thread: Doggie stoke

    by Mofro261
    Replies
    7,669
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    664,411

    332122 Lazy Maisy.

    332122

    Lazy Maisy.
  34. Thread: Doggie stoke

    by Mofro261
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    664,411

    New family member arrived yesterday. ...

    New family member arrived yesterday.
    ...
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    No OG, if I'm skiing and it 0F or below out, the...

    No OG, if I'm skiing and it 0F or below out, the mask is most definitely for me.
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    The number of dead didn't go down. I suggest to...

    The number of dead didn't go down. I suggest to get your understanding of what's being shown in these graphs to go up.

    HINT: LOOK AT THE X AND Y AXIS before gaping your yap again.
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    Just speculation on my part. But if the virus...

    Just speculation on my part. But if the virus only colonizes the nose/throat and doesn't get to the lungs, disease will be mild vs. severe. The last two- how big of an exposure is initally received,...
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    Dengue fever has a massive spread as well, from...

    Dengue fever has a massive spread as well, from asym/pauci-symptomatic to hemmorrhagic fever leading to death. Est 400 million infections each year leading to 100 million people getting actual...
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    &...

    &
    https://upload.wikimedia.org/wikipedia/commons/a/a0/Marlies_van_Baalen_with_%22Kigali%22.jpg
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    There are a couple reports of differential...

    There are a couple reports of differential expression, higher in adults than in children, of the virus receptor ACE2 and the protease TMPRSS within the nasal passages and airways. This could in...
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    All of this. Walked through the zone on Thursday...

    All of this. Walked through the zone on Thursday and it was all peaceful despite what the digitally altered pictures and photo mash ups on Fox would have you believe.
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    Couple months back some guy on...

    Couple months back some guy on Vashon?/Brainbridge? was making one of the sars-2 antigens in his garage and offering up vaccine shots. He was shuttered pretty quick.

    Going rate for recombinant...
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    https://ourworldindata.org/grapher/positive-rate-d...

    https://ourworldindata.org/grapher/positive-rate-daily-smoothed?tab=chart&country=~USA
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    not going to work for stopping viral...

    not going to work for stopping viral replication....
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    One of my co-workers lives on the S. side of Cal...

    One of my co-workers lives on the S. side of Cal Anderson Pk. She had tear gas clouds drifting into her house last week.

    The marches go right past her house and she's been having to time coming...
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    Damn shame that Becky/Karen got the diabetes. ...

    Damn shame that Becky/Karen got the diabetes. That Susan is a bruiser.
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    Harnessing the natural anti-glycan immune...

    Harnessing the natural anti-glycan immune response to limit the transmission of enveloped viruses such as SARS-CoV-2. PLOS PATHOGENS May 21 2020.

    ...
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    Not just Hawaiian shirts, they're desecrating...

    Not just Hawaiian shirts, they're desecrating Ollie & Jerrie and Breakin II.

    -1sUZ5skHps
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    My 3X is 4-5 yrs old. But the sensors in the...

    My 3X is 4-5 yrs old. But the sensors in the watch died ~2-3yrs ago and it's been a fancy step counter ever since. Got a Fenix 6 pro sapphire as a bday gift about a month before these cheap 5X's were...
  50. My 21yro has some of this, not so much gaming but...

    My 21yro has some of this, not so much gaming but heavily into Bike forums and Car forums. On the flip side he also dabbles in bitcoin and has a Robinhood funds acc.... so on second thought he seems...
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